Catalase+Lab+Data

Catalase Lab Data

//**JOVE GARCIA AND JULIE NGUYEN**// //**Data: enzyme concentration**// //**Data: pH 7 and above (basic)**// //**WORD DOCUMENT VERSION**//
 * TRIAL |||| VOLUME OF GAS PRODUCED ||
 * 1 w/ Variation 1 || 4.5 mL || 4.0 mL ||
 * 2 w/ Variation 2 || 2.5 mL || 3.0 mL ||
 * 3 w/ Variation 3 || 1.0 mL || 2.5 mL ||
 * 4 w/ Variation 4 || 2.0 mL || 1.5 mL ||
 * 5 w/ Variation 5 || 1.3 mL || 1.5 mL ||
 * TRIAL |||| VOLUME OF GAS PRODUCED ||
 * 1 @ pH 8 || 2.5 mL || N/A ||
 * 2 @ pH 9 || 2.5 mL || N/A ||
 * 3 @ pH 10 || 3.0 mL || 2.0 mL ||
 * 4 @ pH 11 || 2.5 mL || N/A ||
 * 5 @ pH 12 || 2.5 mL || N/A ||

** Enzyme Variation **

Irteza J. & Cameron E..

d= 2cm ||  1.41 mL    d= 2cm  ||  1.26 mL    d= 2cm  ||  1.26 mL    d= 2cm  ||  1.26 mL    d= 2cm  || d= 2cm ||  1.26 mL    d= 2cm  ||  1.26 mL    d= 2cm  ||  1.09 mL    d= 2cm  ||  0.942 mL    d= 2cm  ||
 * **Variation** ||  1  ||  2  ||  3  ||  4  ||  5  ||
 * **Volume of enzyme (mL)** ||  3  ||  2.5  ||  2  ||  1.5  ||  1  ||
 * **Volume of water** ||  0  ||  0.50  ||  1  ||  1.5  ||  2  ||
 * **[enzyme]** ||  100%  ||  2.5/3*100=83.3%  ||  2/3*100= 66.7%  ||  1.5/3*100=50%  ||  1/3*100=33.3%  ||
 * **Height of Bubbles (First Trial)** ||  1 cm  ||  0.45 cm  ||  0.4 cm  ||  0.4cm  ||  0.4 cm  ||
 * **Volume** ||  3.1416 mL
 * **Height of Bubbles (Second Trial)** ||  1 cm  ||  0.4cm  ||  0.4 cm  ||  0.35 cm  ||  0.3 cm  ||
 * **Volume** ||  3.1416 mL


 * ===== pH 7 and above ===== || ===== Control =====

(No Base)
|| ===== Variation 1 =====

0.1M
|| ===== Variation 2 =====

0.01M
|| ===== Variation 3 =====

0.001M
|| ===== Variation 4 =====

0.0001M
|| ===== Variation 5 =====

0.00001M
||
 * ===== Trial 1 ===== || ===== 6.24 mL ===== || ===== 5.02 mL ===== || ===== 4.40 mL ===== || ===== 3.77 mL ===== || ===== 3.14 mL ===== || ===== 3.77 mL ===== ||
 * ===== Trial 2 ===== || ===== 4.76 mL ===== || ===== 4.40 mL ===== || ===== 3.14 mL ===== || ===== 2.83 mL ===== || ===== 2.51 mL ===== || ===== 3.14 mL ===== ||

By: Martha and Nabeelah

Temperature at 20 o C and below
 * Trial 1 || Temperature: 20.7 o C || Amount of Gas Produced: 3.0 mL || Enzyme Activity: 0.025 mL/s ||
 * Trial 2 || Temperature: 20.1oC || Amount of Gas Produced: 3.0 mL || Enzyme Activity: 0.025 mL/s ||
 * Trial 1 || Temperature: 14.5oC || Amount of Gas Produced: 2.5 mL || Enzyme Activity: 0.021 mL/s ||
 * Trial 1 || Temperature: 10.5oC || Amount of Gas Produced: 0.5 mL || Enzyme Activity: 0.0042 mL/s ||
 * Trial 1 || Temperature: 5.0oc || Amount of Gas Produced: 2.5 mL || Enzyme Activity: 0.021 mL/s ||
 * Trial 1 || Temperature: 1.0oC || Amount of Gas Produced: 2.0 mL || Enzyme Activity: 0.017 mL/s ||

**pH 7 & above (basic) **

 * **Trial ** ||||  **Volume of gas produced **  ||
 * Control (pH 7) || 3.5 mL  || N/A  ||
 * pH 8 || 3.0 mL  || N/A  ||
 * pH 9 || 2.25 mL  || N/A  ||
 * pH 10 || 3.0 mL  || 4.5 mL  ||
 * pH 11 || <span style="display: block; font-family: 'Cambria','serif'; font-size: 12pt; text-align: center;">5.0 mL  || <span style="display: block; font-family: 'Cambria','serif'; font-size: 12pt; text-align: center;">3.5 mL  ||
 * <span style="display: block; font-family: 'Cambria','serif'; font-size: 12pt; text-align: center;">pH 12 || <span style="display: block; font-family: 'Cambria','serif'; font-size: 12pt; text-align: center;">4.0 mL  || <span style="display: block; font-family: 'Cambria','serif'; font-size: 12pt; text-align: center;">N/A  ||

Tahrik and Saad


 * Data: pH 7 and Below (acid) **
 * Variation ||||  Volume of Gas Produced  ||
 * ^  ||  Trial 1  ||  Trial 2  ||
 * 1 neutral || 3.8mL  ||  N/A  ||
 * 2 pH2 || 4.2mL  ||  N/A  ||
 * 3 pH3 || 4.7mL  ||  N/A  ||
 * 4 pH4 || 2.3mL  ||  4.1mL  ||
 * 5 pH5 || 4.2mL  ||  N/A  ||
 * 6 pH6 || 3.2mL  ||  N/A  ||


 * Data: Enzyme Concentration **
 * Variation ||||  Volume of Gas Produced  ||
 * ^  ||  Trial 1  ||  Trial 2  ||
 * 1 3mL enzyme || 6.4mL  ||  5.2mL  ||
 * 2 2.5mL enzyme + 0.5mLwater || 2.4mL  ||  2.6mL  ||
 * 3 2.0mL enzyme + 1.0mL water || 2.1mL  ||  N/A  ||
 * 4 1.5mL enzyme + 1.5mL water || 1.5mL  ||  N/A  ||
 * 5 1.0mL enzyme + 2.0mL water || 0.4mL  ||  N/A  ||

Rajavi and Alyssa


 * Substrate Concentration**
 * Variation || Volume of Bubbles (Trial 1) ||
 * 1. 100% || 4.5mL ||
 * 2. 80% || 3mL ||
 * 3. 60% || 2mL ||
 * 4. 40% || 1.5mL ||
 * 5. 20% || 1.2mL ||


 * pH 7 and below (acidic)**
 * pH variation || Volume of Bubbles (Trial 1) ||
 * Neutral || 4mL ||
 * pH 6 || 3.5mL ||
 * pH 5 || 3mL ||
 * pH 4 || 2mL ||
 * pH 3 || 3mL ||
 * pH 2 || 3.5mL ||

**Sarjini & Annie**
__**Procedure 6: pH 7 and above (basic)**__ || N/A  ||
 * Radius was 1 cm**
 * **pH Level** |||| **Volume of Foam (mL)** ||
 * ^  ||  **Trial 1**  ||  **Trail 2**  ||
 * **7 (control)** ||  7.2  ||  5.96  ||
 * **8** ||  6.6  ||  N/A  ||
 * **9** ||  5.7  ||  N/A  ||
 * **10** ||  5.7  ||  N/A  ||
 * **11** ||  5.3  ||  N/A  ||
 * **12** ||  5.0
 * __Procedure 1: Temperature 20 degrees Celsius and above__**
 * **Trial** || **Temperature (degrees Celsius)** || **Volume of foam (mL****)** ||
 * 1 || 23 || 6.8 ||
 * 2 || 23 || 5.338 ||
 * 1 || 25 || 5.966 ||
 * 2 || 25 || N/A ||
 * 1 || 30 || 6.8 ||
 * 2 || 30 || N/A ||
 * 1 || 35 || 7.222 ||
 * 2 || 35 || N/A ||
 * 1 || 40 || 9.106 ||
 * 2 || 40 || N/A ||
 * 2 || 40 || N/A ||

= = = = = = = = = = = = =Nathan and Aynul=

Substrate Concentration

 * Variation |||| volume of gas produced ||
 * Substrate concentration || Trial 1 || Trial 2 ||
 * 100% || 4.5mL || 3mL ||
 * 80% || 2.5mL || 3mL ||
 * 60% || 3mL || 2.5mL ||
 * 40% || 1.5mL || 2mL ||
 * 20% || 0.5mL || 0.5mL ||

pH 7 and above (base)

 * Variation |||| volume of gas produced ||
 * pH || Trial 1 || Trial 2 ||
 * 7 || 4mL || 3.5mL ||
 * 8 || 2.5mL || 3mL ||
 * 9 || 2.5mL || 2.5mL ||
 * 10 || 1mL || 3mL ||
 * 11 || 1.5mL || 3mL ||
 * 12 || 3mL || 2mL ||

pH below (Acidic) Temperature 20 C and above
 * Nejaha and Anne **
 * Trial || Amount of gas ||
 * 1 || pH 2 1.5 mL ||
 * 2 || pH 2 2 mL ||
 * 1 || pH 3 N/A ||
 * 2 || pH 3 2 mL ||
 * 1 || pH 4 2.5mL ||
 * 2 || pH 4 2 mL ||
 * 1 || pH 5 2 mL ||
 * 2 || pH 5 3 mL ||
 * 1 || pH 6 2.5mL ||
 * 1 || pH 7 5 mL ||
 * Temperature || Trial || Amount of foam ||
 * 20 C || 1 || 2.8 mL ||
 * 27 C || 1 || 3.8 mL ||
 * 33 C || 1 || 6.3 mL ||
 * 38 C || 1 || 7.8 mL ||
 * 42 C || 1 || 7.8 mL ||


 * //Joey and Alexis//**

__**Substrate Concentration**__
 * Variation || Volume of Gas Produced ||
 * 1 - 100% || 6mL of bubbles ||
 * 2 - 80% || 6.5mL of bubbles ||
 * 3 - 60% || 1mL of bubbles ||
 * 4 - 40% || 1.75mL of bubbles ||
 * 5 - 20% || 1mL of bubbles ||

__**pH Below (Acidity)**__


 * pH Varitation || Volume of Gas Produced ||
 * 2 || 4.5mL of bubbles ||
 * 3 || 4mL of bubbles ||
 * 4 || 3mL of bubbles ||
 * 5 || 2.5mL of bubbles ||
 * 6 || 4mL of bubbles ||
 * 7 (neutral) || 6.5mL of bubbles ||

//** KATHRYN & SHANUKHA **// Procedure Two: Temperature below 20 Degrees Celsius

Substrate: Clear, transparent liquid || White foam forming on top of a beige, opaque liquid. || 23mL Foam high formed ||
 * Trial || Before || During || After ||
 * 1 (@ 23 degrees) || Enzyme: Translucent, brown, liquid, some suspension
 * 2 (@ 15 degrees) || No Change || No change || 17mL Foam high formed ||
 * 3 (@ 10 degrees) || No Change || No change || 4mL Foam high formed ||
 * 4 (@ 6 degrees) || No Change || No change || 3.5mL Foam high formed ||
 * 5 (@ 1 degrees) || No Change || No change || 10mL Foam high formed (first time) 6mL of foam high formed (second time) ||

Procedure Five: pH below 7 Substrate: Clear, transparent liquid || White foam forming on top of a beige, opaque liquid. || 18mL Foam high formed ||
 * Trial || Before || During || After ||
 * 1 (pH 7) || Enzyme: Translucent, brown, liquid, some suspension
 * 2 (HCl @ 10^-5M) || No Change || No change || 6mL Foam high formed ||
 * 3 (HCl @ 10^-4M) || No Change || No change || 15mL Foam high formed ||
 * 4 (HCl @ 10^-3M) || No Change || No change || 13mL Foam high formed ||
 * 5 (HCl @ 0.01M) || No Change || No change || 16mL Foam high formed ||
 * 6 (HCl @ 0.10M) || No Change || No change || 14mL Foam high formed ||